Journal: Oncotarget

Article Title: A lesson for cancer research: placental microarray gene analysis in preeclampsia

doi:

Figure Lengend Snippet: Summary of microarray studies in preeclampsia

Article Snippet: Hoegh 2010 Denmark , 11 PE 18 ctrl , , , , Cs/vag. , Maternal side, center of cotyledons, stored differently, Pooled RNA. , Affymetrix HG-U133A Gene Chip , 12 up and 9 down in PE vs. ctrl. , Bradykinin B1 receptor, 14-3-3, INHBA, LEP , Placentation, oxidative stress, inflammation.

Techniques: Microarray, Sampling, Dissection, Expressing, Cell Function Assay

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: High-Content Imaging Assays on a Miniaturized 3D Cell Culture Platform

doi: 10.1016/j.tiv.2018.02.014

Figure Lengend Snippet: The Z’ factors and the CV values of the HCI assays performed on the microarray chip platform.

Article Snippet: For robust cell spot attachment and miniaturized 3D cell culture on the microarray chip platform, micropillar chips were coated with 0.01% PMA-OD followed by spotting 60 nL volume of a mixture of 0.003% PLL and 16.66 mM BaCl 2 onto the micropillar chips using a microarray spotter (S+ microarrayer from Samsung Electro-Mechanic Co. or SEMCO).

Techniques: Microarray

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: High-Content Imaging Assays on a Miniaturized 3D Cell Culture Platform

doi: 10.1016/j.tiv.2018.02.014

Figure Lengend Snippet: Representative images from 72 h-incubated micropillar chips with 3D-cultured Hep3B cells, exposed to menadione (0.7 – 177 μM) for 48 h, and stained with the four fluorescent dyes: (A) TMRM for mitochondrial impairment, (B) Hoechst 33342 for DNA damage, (C) mBCl for changes in intracellular glutathione level, and (D) calcein AM for cell membrane integrity. The dose-dependent effect of menadione for all four assays was clearly observed from the microarray images obtained.

Article Snippet: For robust cell spot attachment and miniaturized 3D cell culture on the microarray chip platform, micropillar chips were coated with 0.01% PMA-OD followed by spotting 60 nL volume of a mixture of 0.003% PLL and 16.66 mM BaCl 2 onto the micropillar chips using a microarray spotter (S+ microarrayer from Samsung Electro-Mechanic Co. or SEMCO).

Techniques: Incubation, Cell Culture, Staining, Membrane, Microarray

Journal: PLoS ONE

Article Title: Dysregulation of IFN System Can Lead to Poor Response to Pegylated Interferon and Ribavirin Therapy in Chronic Hepatitis C

doi: 10.1371/journal.pone.0019799

Figure Lengend Snippet: The time frame of liver biopsy, microarray analysis, therapeutic period, observation period after combination therapy, and the judging of clinical outcome is shown.

Article Snippet: DNA oligonucleotide probes were synthesized onto a DNA microarray chip called Genopal (Mitsubishi Rayon) in order to detect the 237 genes (200 genes on Chip1 and 37 genes on Chip2) related to the innate immune response.

Techniques: Microarray